You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

prkD [2017-06-06 09:01:34]

protein kinase D

locus

BSU02030

6.36

29.91 kDa

protein length

256 aa Sequence Blast

gene length

768 bp Sequence Blast

function

unknown

product

protein kinase D

essential

2.7.11.1

synonyms

ybdM

Outlinks

KEGG BsubCyc SubtiList UniProt Expression Browser

Genomic Context

Hide small RNAs Zoom:

categories

[category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]

[category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]

Gene

Coordinates

223,219 → 223,989

The protein

Catalyzed reaction/ biological activity

ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot)

phosphorylates [protein|047CE33C0CC127DAD15D68D63843DF89F93ED3BF|DegS] on Ser-76 (in vitro) [Pubmed|21304896]

Protein family

[SW|protein kinase] domain (according to Swiss-Prot)

Modification

autophosphorylated on Thr-174 (''in vitro'') [Pubmed|20389117]

Biological materials

Mutant

MGNA-B958 (ybdM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1957 NBRP B. subtilis, Japan]

GP578 (aphA3), available in the [SW|Stülke] lab

Expression vector

pGP390 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Stülke] lab

for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172]: pGP821, available in [SW|Stülke] lab

for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP1407, available in [SW|Stülke] lab

lacZ fusion

pGP830 (in [protein|search|pAC7]), available in [SW|Stülke] lab

References

20389117,12406230,21304896,24731262,25278935